2016 2015 

Chih-Pin Chuu褚志斌博士      

Int. J. Mol. Sci.2015, 16(1), 677-690; doi:10.3390/ijms16010677

Reduced 5-Methylcytosine Level as a Potential Progression Predictor in Patients with T1 or Non-Invasive Urothelial Carcinoma  ijms-logo

This study aims to elucidate the level of DNA methylation in urothelial carcinomas (UCs) using 5-methylcytosine (5-MeC) immunohistochemistry (IHC). We examined the relationship among 5-MeC levels, DNA (cytosine-5)-methyltransferase 1 (DNMT1) immunostaining levels, and clinicopathologic features. Tissue samples included 23 normal urothelia and 150 urothelial neoplasia, which comprised 40 non-invasive and 110 invasive UCs. The levels of 5-MeC and DNMT1 were assessed based on their immunoreactivities and then divided into low and high levels. ………….detail

Chih-Pin Chuu褚志斌博士

Volume 369, Issue 1, 1 December 2015, Pages 103–111          1-s2_0-S0304383515X00243-cov150h

Androgen receptor inhibits epithelial–mesenchymal transition, migration, and invasion of PC-3 prostate cancer cells

Bone metastasis is very common in prostate cancer (PCa) and causes severe pain. PC-3 is an androgen receptor (AR)-negative PCa cell line with high metastatic potential established from PCa bone metastasis. We observed that re-expression of AR, which is located in the cytoplasm in the absence of androgen, ………….detail

Jun-Yang Liou劉俊揚博士

Volume 201, 15 December 2015, Pages 441–4481-s2_0-S0167527315X00210-cov150h

Rho-associated kinase inhibitors promote the cardiac differentiation of embryonic and induced pluripotent stem cells

Rho-associated kinase (ROCK) plays an important role in maintaining embryonic stem (ES) cell pluripotency. To determine whether ROCK is involved in ES cell differentiation into cardiac and hematopoietic lineages, we evaluated the effect of ROCK inhibitors, Y-27632 and fasudil on murine ES and induced pluripotent stem (iPS) cell differentiation. ………….detail

Ing-Ming Chiu邱英明博士

Volume 24, Issue 24: December 15, 2015

Activation of FGF1B Promoter and FGF1 Are Involved in Cardiogenesis Through the Signaling of PKC, but Not MAPK

Heart disease is the leading cause of human death in the 21st century. Heart transplantation is a promising way to treat this. Because donor resources are limited, cell-based therapy has been developed as an alternative. Therefore, genes that trigger cardiogenesis could have potential in the treatment of heart disease. Fibroblast growth factor 1 (FGF1) is reported to stimulate cardiomyocyte proliferation under conditions of myocardial infarction, ………….detail

December 24, 2015 Journal of Visualized Experiments, (106),e53265,doi:10.3791/53265.

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)JoVE

The immunomodulatory properties of multilineage human mesenchymal stem cells (MSCs) appear to be highly relevant for clinical use towards a widerangeof immune-related diseases. Mechanisms involved are increasingly being elucidated and in this article, we describe the basic experiment to assess MSC immunomodulation by assaying for suppression of effector leukocyte proliferation. Representing activation, leukocyte proliferation can be assessed by a number of techniques, and we describe in this protocol the use of the fluorescent cellular dye carboxyfluorescein succinimidyl ester (CFSE) to label leukocytes with subsequent flow cytometric analyses……………detail

Volume:5,1-13, Stem Cell Reports. Sept. 8 2015.
Interleukin-25 Mediates Transcriptional Control of PD-LI via STAT3 in Multipotent Human Mesenchymal Stromal Cells(hMSCs) to Suppress TH17 Responses.
Multipotent human mesenchymal stromal cells (hMSCs) harbor immunomodulatory properties that are therapeutically relevant. One of the most clinically important populations of leukocytes is the interleukin-17A (IL-17A)-secreting T (Th17) lymphocytes………detail

Stem Cell Reports. 2013 Jul 25;1(2):139-51.
Multipotent Human Mesenchymal Stromal Cells Mediate Expansion of Myeloid-Derived Suppressor Cells via Hepatocyte Growth Factor/c-Met and STAT3.
Multipotent human mesenchymal stromal cells (hMSCs) harbor immunomodulatory properties that are therapeutically relevant. One of the most clinically important populations of leukocytes is the interleukin-17A (IL-17A)-secreting T (Th17) lymphocytes. However,mechanisms of hMSC and Th17 cell interactions are incompletely resolved. We found that, along with Th1 responses, hMSCs strongly suppressed Th17 responses and this required both IL-25—also known as IL-17E—as well as programmed death ligand-1 (PD-L1), a potent cell surface ligand for tolerance induction. ……..detail
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